Modifying Enzymes
Worthington Biochemical Corporation COLLAGENASE TYPE 4 50 MG
Collagenase, Type 4 (Code: CLS-4), which is filtered through a 0.22 micron membrane and lyophilized in vials.
New England Biolabs, Inc. EnGen Cas9 NLS, S. pyogenes – 2000 pmol
EnGen Spy Cas9 NLS, is an RNA-guided endonuclease that catalyzes site- specific cleavage of double stranded DNA. The location of the break is within the target sequence 3 bases from the NGG PAM (Protospacer Adjacent Motif) ). The PAM sequence, NGG, must follow the targeted region on the opposite strand of the DNA with respect to the region complementary sgRNA sequence. EnGen Spy Cas9 NLS contains Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein.
Sigma Aldrich Fine Chemicals Biosciences M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis | 9068-38-6 | MFCD00283754 | 40KU
M-MLV Reverse Transcriptase Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis | 9068-38-6 | MFCD00283754 | 40KU
GeneCopoeia BIOTIN PROTEIN LIGASE
Biotin Protein Ligase - 40 ug: 2 vials of 20 ug each (200,000 Units)
Sigma Aldrich Fine Chemicals Biosciences Chitinase from Trichoderma viride lyophilized powder, >=600 units/g solid | MFCD00130771 | 25UN
Chitinase from Trichoderma viride lyophilized powder, >=600 units/g solid | MFCD00130771 | 25UN
Sigma Aldrich Fine Chemicals Biosciences alpha-Amylase from Aspergillus oryzae >=150 units/mg protein (biuret) | MFCD00081319 | 1MU
alpha-Amylase from Aspergillus oryzae >=150 units/mg protein (biuret) | MFCD00081319 | 1MU
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC3915858 A-AMYLASE FROM HUMAN SALIVA
Abcam Recombinant human Catalase protein, 100UG
Recombinant human Catalase protein is a Human Full Length protein, in the 1 to 527 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Protein/ Peptide/ Enzyme Synonyms: Catalase, CAT
Conjugation: Unconjugated
The product is subject to the following: Abcam Restricted Use Statement
Worthington Biochemical Corporation ELASTASE LYOPHILIZED 1GM
Two times crystallized, (Code: ESL), supplied as a dialyzed, lyophilized powder. The enzyme should be 0.22 micron filtered after reconstitution and prior to use. Suitable for the isolation of Type II lung cells. 2594-83-7
Worthington Biochemical Corporation DEOXYRIBONUCLEASE I RN 100UN
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied as a solution at approximately 2 Kunitz units per microliter (approximately 1 mg/ml) containing 50% glycerol and 1 mM calcium chloride. 9003-98-9
Worthington Biochemical Corporation STEMXYME 1 COLLAGENAS 50MG
A specialized combination of Animal Origin-Free Clostridium histolyticum collagenase (Code: CLSAFB) and animal origin free Bacillus polymyxa neutral protease with a minimum of 250 CLS units and 1,000 caseinase units per mg dry weight. Designed for stem cell and other primary cell isolations and bioprocessing applications where introduction of potential animal derived pathogens must be prevented. 2593-92-3
Enzo Life Sciences HDAC8 (human), (recombinant) (100 U)
Alternative name: Histone deacetylase 8. Formulation: Liquid. In 10mM TRIS, pH 7.5, containing 100mM NaCl, 3mM MgCl2 and 10% glycerol. Source: Produced in E. coli. Recombinant HDAC8 from human cDNA (377 aa). Long Term Storage: -80°C.
Supply Solutions Liberase™ TL Research Grade, pkg of 10 mg (2x5 mg)
The most commonly used method for decellularization procedures, such as tissue dissociation and cell harvesting, is based on the use of proteolytic enzymes. Proteases disrupt the extracellular matrix of tissue to allow the release of individual cells or the harvesting of cultured cells for transfer. The goal of cell isolation is to maximize the yield of dissociated cells that are viable and functionally active.