Modifying Enzymes
ABCAM LTD URB754, Monoacylglycerol lipase inhibitor, 50MG
MW 266.3 g/mol, Purity >98%. Reported potent and non-competitive monoacylglycerol lipase (MAGL) inhibitor (IC₅₀ = 200 nM). Inhibits FAAH (IC₅₀ = 32 μM) and binds weakly to the rat CB₁ receptor (IC₅₀ = 3.8 μM). Shows vasodilatory activity.
The product is subject to the following: Abcam Restricted Use Statement
APEXBIO TECHNOLOGY LLC Phusion high-fidelity DNA polymerase, 100U
Phusion high-fidelity DNA polymerases are composed of a DNA-binding domain fused to a Pyrococcus-like proofreading polymerase. Because of this, Phusion DNA polymerases produce PCR products with very high speed and accuracy. Also, Phusion DNA polymerases are tolerant of various inhibitors, allowing for robust amplification with minimal optimization. Phusion DNA Polymerase is an ideal choice for cloning and can be used for long or difficult (GC-rich) amplicons. Phusion high-fidelity DNA polymerases have become the choice for even demanding applications like massively parallel high-throughput sequencing of whole genomes. Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC PreScission Protease (PSP), 500U (1U/uL). Cas: MFCD: NA. Specifically recognize and cleave the Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro peptide in fusion proteins.
PreScission Protease is a fusion protein of human rhinovirus 3C protease and glutothione S trasferase (GST). PreScisson protease can specifically recognize the Leu-Glu-Val-Leu-Phe-Gln/Gly-Pro sequence and cleaves between the Gln and Gly residues. Concentration: 2U/L Other sizes are also available. Please inqury us for quote.
Apexbio Technology LLC EZ Cap ER-EGFP Probe mRNA (m1Ψ) 5x1mg (1mg/mL)
ER-EGFP mRNA encodes a fusion protein comprising an endoplasmic reticulum (ER) targeting signal sequence and enhanced green fluorescent protein (EGFP) EGFP is a widely used fluorescent marker protein for biological imaging By fusing EGFP to the ER signal sequence dynamic changes in the ER can be observed in real-time in live cells making it particularly useful for studying the structure function and interactions of the ER with other cellular organelles ER-EGFP s efficient fluorescent properties and specific localization to the ER position it as an ideal tool for studying ER-related processes The EZ Cap ER-EGFP Probe mRNA (m1 ) is provided at 1 mg/mL specifically designed for targeted expression of a green fluorescent protein label at the ER This mRNA is synthesized through co-transcriptional capping resulting in a Cap 1 structure The incorporation of m1 modifications poly(A) tail significantly enhances mRNA stability and lifetime both in vitro and in vivo
Apexbio Technology LLC EZ Cap Mito-EGFP Probe mRNA (m1Ψ) 5x1mg (1mg/mL)
Mito-EGFP mRNA encodes a fusion protein composed of a mitochondrial localization sequence fused with enhanced green fluorescent protein (EGFP) Through fusion of EGFP with a mitochondrial-targeting signal it allows real-time observation of mitochondrial dynamics in living cells aiding in revealing mitochondria s key roles in energy metabolism signal transduction and apoptosis Due to its high fluorescence efficiency and mitochondria-specific localization Mito-EGFP simplifies mitochondrial visualization facilitating a clearer understanding of mitochondrial function and behavior in cells EZ Cap Mito-EGFP Probe mRNA (m1 ) is provided at 1 mg/mL and is specifically optimized for mitochondria-specific expression of green fluorescent protein labeling This mRNA is synthesized through co-transcriptional capping resulting in a Cap 1 structure The incorporation of m1 modifications poly(A) tail significantly enhances mRNA stability and lifetime both in vitro and in vivo
Apexbio Technology LLC EZ Cap OVA mRNA (m1Ψ) 5x1mg (1mg/mL)
Ovalbumin (OVA) mRNA encodes a egg white glycoprotein with a molecular weight of approximately 45 000 daltons It can induce moderate immunogenicity in organisms and is commonly used as an immunogen in immunological experiments such as establishing animal models for hypersensitivity asthma etc EZ Cap OVA mRNA (m1 ) is provided at 1 mg/mL capped by co-transcriptional capping with a Cap 1 structure and polyadenylated Modified m1 incorporation reduces the host cell immune response and enhances mRNA stability and translation efficiency
Enzo Life Sciences PARP-1 (catalytic domain) (human), (recombinant) (His-tag) (20µg)
Alternative name: Poly(ADP-ribose) polymerase 1. Purity: ≥90% (SDS-PAGE). MW: ~46kDa. Formulation: Liquid. In 50mM TRIS-HCl, pH 7.5, containing 300mM sodium chloride, 250mM imidazole, 2mM β-mercaptoethanol, 0.2% NP-40 and 10% glycerol. Source: Produced in SF21 insect cells. Human PARP-1 (aa 656-1014) is fused to a C-terminal His-tag. Produced in a baculovirus expression system. Long Term Storage: -80°C. Handling: Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -80°C.
Enzo Life Sciences Matriptase (human), (recombinant) (His-tag) (250U)
Alternative name: MT-SP1, Membrane type serine protease 1, ST14, Suppression of tumorigenicity protein 14, TADG-15, Tumor associated differentially expressed gene 15 protein, SNC19. Concentration: 100nM. Purity: ≥98% (SDS-PAGE). MW: ~25kDa. Formulation: Liquid. In 100mM TRIS, pH 8.0, containing 200mM sodium chloride, 1mM β-mercaptoethanol and 10% glycerol with a recommended assay buffer of 50mM HEPES, pH 7.4, 150mM NaCl, with 5mg/ml BSA. Source: Recombinant human matriptase (aa 596-855) fused at the N-terminus to a His-tag. Long Term Storage: -80°C. Use/Stability: Stable for at least 6 months when stored at -20°C. Do not store diluted solutions. Handling: Avoid freeze/thaw cycles.
Zymo Research Corporation Zymolyase Ultra 10000 U
Digestion of yeast and fungal cell walls is necessary for many experimental procedures. Lytic enzymes like Zymolyase Lyticase Glucanase or Chitinase are routinely used for digestion of cell walls. Distinguished from conventional lytic enzymes Zymolyase Ultra is a novel formulation of enzyme that is optimized for efficient yeast cell wall digestion. It contains enzymes that can degrade several different components of yeast cell walls. It is extensively purified using a novel DNA/RNA removal technology that results in extremely low nucleic acid contamination. It is suitable for qPCR and sequencing-based assays where low DNA/RNA contamination is critical.
Bioworld Alkaline Phosphatase in 50% Glycerol, 5000 Unit(s)
Alkaline Phosphatase in 50% GlycerolSpecifications:Source: Calf Intestine.Supplied in 50% GlycerolActivity: 3000 - 4000 U/mg proteinApplication: as an indicator enzyme when conjugted to proteins such as antibodies, avidin etc. Suitable also for western blot analysis.Unit definition: the amount of enzyme catalyzing the liberation of 1 μmole of p-nitrophenyl phosphate per minute at 37° C, pH 9.8 in diethanolamine buffer, 405nm.If assayed at 25°C in glycine buffer, pH 9.6, then, 1 glycine unit= 3DEA units
Bioworld Beta-Glactosidase, 5000 Unit(s)
Beta-Galactosidase Enzyme found in lac+ bacterial cells that catalyzes the cleavage of -linked terminal galactose-containing substratesLyophilized powderSource: E. coliConjugation gradeActivity (37°C):800-1000U/mg proteinUnit definition: One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH7.3 at 37°C, 410nm.Aggregation (HPLC): <3.0%Application: Used as an indicator enzyme conjugated to proteins such as antibodies.
![Bioworld Cellulase RS [Onozuka RS], 10 g](https://assets.fishersci.com/TFS-Assets/CCG/product-images/50198966.jpg-250.jpg)
Bioworld Cellulase RS [Onozuka RS], 10 g
From mutant Trichoderma viride derived from the parent strain for Cellulase Onozuka R-10. Cellulase RS contains a high activity of decomposing natural celluloses. Can be used to obtain protoplasts and dissolve cell walls in a wide range of plants.* Soluble in water* Optimum pH range 4.0-5.0* Optimum temperature range 50° to 60°CUsually ships within 24 hours.This product is sold for laboratory research use only.
Bioworld DNase I (Bovine Pancreas), 100 KU
DNase I DNase I is an endonuclease derived from bovine pancreas that will degrade double-stranded DNA in the presence of divalent cations, producing 3'-OH oligonucleotides. Mg+2 in the reaction solution causes the enzyme to produce nicks in double-stranded DNA, while in the presence on Mn+2, DNase I cleaves both strands of the DNA. DNase I is useful in nick translation for introducing single-stranded nicks that serve as primer sites for initiation of DNA synthesis and for cloning random DNA fragments by cleaving double-stranded DNA. It is also used in purifying RNA from cells or transcription mixtures. Specifications:CAS: 9003-98-9EC: 232-667-0Source: Bovine pancreasActivity: ≥10000 U/mg.Ultra Pure GradeOur DNase I is guaranteed free of RNase contaminants.
Crescent Chemical Co Inc CELLULOSE ;ONOZUKA R-10+
Cellulase »Onozuka R-10« from Trichoderma viride ca. 1 U/mg A multi-component enzyme system (1). Although the preparation has high cellulase activity, it still contains hemicellulases, and it degrades mannans, xylans, galactomannans, pectins and other polysaccharides. Widely used for the isolation of prot
Crescent Chemical Co Inc CELLULOSE ;ONOZUKA RS+
Cellulase »Onozuka RS« from Trichoderma viride ca. 2 U/mg A multi-component enzyme system (1). Although the preparation has high cellulase activity (EC 3.2.1.4), it still contains hemicellulases, and it degrades mannans, xylans, galactomannans, pectins and other polysaccharides. Contains about three tim